Progress towards cell-mediated gene transfer in zebrafish
نویسندگان
چکیده
منابع مشابه
Specific RNAi mediated gene knockdown in zebrafish cell lines.
Here we demonstrate highly efficient RNA interference in ZFL, SJD and ZF4 cell lines derived from adult and embryonic zebrafish Danio rerio. Microinjection of siRNAs resulted in silencing in almost 100% of cells while transfection using cationic liposomes led to silencing in 30%. Use of siRNAs against zebrafish lamin A, lamin B2 and the motor protein Eg5, led to knockdown of the target genes wi...
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ژورنال
عنوان ژورنال: Briefings in Functional Genomics and Proteomics
سال: 2002
ISSN: 1473-9550,1477-4062
DOI: 10.1093/bfgp/1.2.131